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Legend of the Sack Stamp 500
victor809 Offline
#151 Posted:
Joined: 10-14-2011
Posts: 23,866
opelmanta1900 wrote:
Well can you tell me why I've got 5 plants going from seed, from the same batch of bud, and 3 of those plants are days away from harvest and the other 2 don't have a single orange hair yet?



I had to look up what the hell you meant by "orange hair"...
Sounds like you've just got an example of some getting more nutrition than others... or less toxins. I don't know what the hell it takes to grow a plant, much better off telling you how a regulatory cascade works in protein expression. :)

Are they in different pots? or all in the same pot?
opelmanta1900 Offline
#152 Posted:
Joined: 01-10-2012
Posts: 13,954
That's a serious question... i understand it's because of phenotypic plasticity, just don't understand how it can make living things with the Same genetics so different...
opelmanta1900 Offline
#153 Posted:
Joined: 01-10-2012
Posts: 13,954
victor809 wrote:
I had to look up what the hell you meant by "orange hair"...
Sounds like you've just got an example of some getting more nutrition than others... or less toxins. I don't know what the hell it takes to grow a plant, much better off telling you how a regulatory cascade works in protein expression. :)

Are they in different pots? or all in the same pot?

5 plants, 5 different pots, but identical... light source is identical... nutrients are identical... the leaf shape, plant heights are virtually identical... but once they started flowering it was clear that one was incredibly different than the others and another one - while very similar looking flower - is growing much larger flowers but maturing much slower than the others... much much slower... it's weird....
banderl Offline
#154 Posted:
Joined: 09-09-2008
Posts: 10,153
opelmanta1900 wrote:
Well can you tell me why I've got 5 plants going from seed, from the same batch of bud, and 3 of those plants are days away from harvest and the other 2 don't have a single orange hair yet?



Seeds are not going to be genetically identical.
You will get a very tremendous difference in plants when growing from seed
That is one of the main reasons for cloning.
There is also a chance that those two plants are male.
opelmanta1900 Offline
#155 Posted:
Joined: 01-10-2012
Posts: 13,954
Not males... they started flowering 1/15... if i can't spot a male after that long, i gotta stick to other things... and i just do this for fun, not trying to grow anything super.. love growing everything from seed - peppers, tomatoes, lettuce, mustard, chard, melons, you name it... not a fan of clones and cuttings... no character...
victor809 Offline
#156 Posted:
Joined: 10-14-2011
Posts: 23,866
opelmanta1900 wrote:
That's a serious question... i understand it's because of phenotypic plasticity, just don't understand how it can make living things with the Dane genetics so different...


So... "phenotypic plasticity" is a funny term. It has a really really broad application.

you know the difference between phenotype and genotype?
Genotype is your genetics. Your genotype is the blueprints... it never changes.
Phenotype is the expression of the genetics... ie "black hair" "blue eyes" "short" "tall".... these are all driven by the genetics, but there may be different genes expressed at different times which may change your phenotype.

The interesting examples of "phenotypic plasticity" are large scale changes to an organism due to huge sequences of new genes being expressed....
- in bacteria, the upregulation of an entire new sugar metabolism pathway (galactose) beause of the prevalence of that sugar in the environment
- I believe there are certain temperature dependent changes.. I think bear hibernation may be an example, as entire metabolic pathways change due to environmental changes (cold)
- The growth of a "winter pelt" which is then shed is a phenotypic pathway change. Those are different genes that are turning on or off
- I believe birds molting from one color as youths to a different color as adults is a phenotypic plasticity ....

But... that same cool term can be used for really basic, dumb ideas we all know about.
malnutrition....
Don't feed some things and that stunts development. If you're depending on your plants to all grow at the same rate they have to get the same nutrition... technically speaking, if one is not getting the same amount of nutrition, then the gene pathways for maturation may trigger later. Technically that's a different phenotype.... but not in the more interesting way that we think of... more in a "take two twins, raise one on meat and the other on bread and water and no sun.. see how they turn out"

opelmanta1900 Offline
#157 Posted:
Joined: 01-10-2012
Posts: 13,954
So are a brother and sister considered genetically the same but phenotypically different?
victor809 Offline
#158 Posted:
Joined: 10-14-2011
Posts: 23,866
banderl wrote:
Seeds are not going to be genetically identical.
You will get a very tremendous difference in plants when growing from seed
That is one of the main reasons for cloning.
There is also a chance that those two plants are male.


I don't know a damn thing about botany...
Wouldn't seeds from the same flower be identical?

If they're seeds from 2 different flowers, then they can be fertilized by two completely different other plants, could have completely different genetic makeup.

But the same flower (and here's where my gap in botany is)... I don't know if each seed originated from a combination of two different instances of pollen... or whether a cluster of seeds from one flower are all the result of a single fertilization instance, and then lots of "twinning"....

If it's the former, then you will have genetically different species with each seed (fraternal twins) if it's the latter then you should have identical genotypes...
victor809 Offline
#159 Posted:
Joined: 10-14-2011
Posts: 23,866
opelmanta1900 wrote:
So are a brother and sister considered genetically the same but phenotypically different?


genetically different
Brother and sister each come from an independent mixing of chromosome pairs from each parent. If you imagine each of the pairs having a "left" and "right" side (they don't, this is just for discussion), each zygote (sperm/egg) is a random selection of right and left sides for each of the pairs (I think there are 32, if my memory is correct)... that's a ton of different combinations. That randomization occurs for both the sperm and egg from each parent.

The resulting offspring is the pairing of the randomly chosen "left/right side".

So each independent pairing can have a vastly different set of genetic combinations
opelmanta1900 Offline
#160 Posted:
Joined: 01-10-2012
Posts: 13,954
so do you think it would it stand to reason that each seed is also likely genetically different even if they come from the same plant?
victor809 Offline
#161 Posted:
Joined: 10-14-2011
Posts: 23,866
opelmanta1900 wrote:
so do you think it would it stand to reason that each seed is also likely genetically different even if they come from the same plant?


I don't know the fertilization process within a single flower...

Let me look a couple things up
MACS Offline
#162 Posted:
Joined: 02-26-2004
Posts: 79,833
It's important to separate the males and females, so the buds don't get seeds.

You know 6 plants is the limit, right? Why just 5? Anxious
victor809 Offline
#163 Posted:
Joined: 10-14-2011
Posts: 23,866
Looks like a number of ovules per flower
https://en.wikipedia.org/wiki/Ovule
Each ovule will have a different randomization of haploid chromosomes...
Each ovule will become diploid when fertilized with a pollen haploid.

So technically, each of the seeds should have a different genetic makeup, even though they likely share the same parents.

Same way brothers/sisters will have different genetic makeup. Different randomized selection of haploid chromosomes were paired to produce them.

Check out mendel's work to see how the combination of chromosomes can lead to specific statistically expected outcomes
opelmanta1900 Offline
#164 Posted:
Joined: 01-10-2012
Posts: 13,954
MACS wrote:
It's important to separate the males and females, so the buds don't get seeds.

You know 6 plants is the limit, right? Why just 5? Anxious

had to cull a runt early on... all 5 seeds were female... possibly from a feminized plant...
Speyside Offline
#165 Posted:
Joined: 03-16-2015
Posts: 13,106
So the reason is genetic variation. Some of the seeds probably were pollinated from different sources.
MACS Offline
#166 Posted:
Joined: 02-26-2004
Posts: 79,833
opelmanta1900 wrote:
had to cull a runt early on... all 5 seeds were female... possibly from a feminized plant...


LOL
opelmanta1900 Offline
#167 Posted:
Joined: 01-10-2012
Posts: 13,954
Speyside wrote:
So the reason is genetic variation. Some of the seeds probably were pollinated from different sources.

Most growers don't keep any males around, and if they do usually just one unless they're specifically trying to develop new stains...

Speyside Offline
#168 Posted:
Joined: 03-16-2015
Posts: 13,106
Got it.
Speyside Offline
#169 Posted:
Joined: 03-16-2015
Posts: 13,106
Though wouldn't genetic variation still be a viable reason?
Speyside Offline
#170 Posted:
Joined: 03-16-2015
Posts: 13,106
I am specifically thinking about apple trees. They are grafted due to seeds producing many different types of apple trees not similar to the parents.
victor809 Offline
#171 Posted:
Joined: 10-14-2011
Posts: 23,866
You will still have genetic variation with only one male.
The plants have chromosome pairs, so you will get variation as one half of a pair or another is selected to be the gamete.

Each half of a pair may have different genetics, which is where dominant and recessive traits come in...
danmdevries Offline
#172 Posted:
Joined: 02-11-2014
Posts: 17,451
victor809 wrote:
genetically different
Brother and sister each come from an independent mixing of chromosome pairs from each parent. If you imagine each of the pairs having a "left" and "right" side (they don't, this is just for discussion), each zygote (sperm/egg) is a random selection of right and left sides for each of the pairs (I think there are 32, if my memory is correct)... that's a ton of different combinations. That randomization occurs for both the sperm and egg from each parent.

The resulting offspring is the pairing of the randomly chosen "left/right side".

So each independent pairing can have a vastly different set of genetic combinations


Transposed your numbers
23 pair 46 total.


ETA: Also, IIRC, each female reproductive thing (can't remember the name. follicle?) will have its own set of half the chromosomes, as will each pollen particle. So the resulting seeds will have randomized makeup, not all copies.
victor809 Offline
#173 Posted:
Joined: 10-14-2011
Posts: 23,866
danmdevries wrote:
Transposed your numbers
23 pair 46 total


dammit... sorry. Been close to 20 years since I thought of this stuff
danmdevries Offline
#174 Posted:
Joined: 02-11-2014
Posts: 17,451
Opel, it's been a very long time but in my experience, even if you buy the fancy seeds autoflower, feminized etc you're not going to get all the same plant - for reasons noted above when working with seeds.

If you want everything to grow the same, you need to keep a mother and clone.

Keep the two in flower cycle with your lighting and nutrients and hope for the best.
RMAN4443 Offline
#175 Posted:
Joined: 09-29-2016
Posts: 7,683
opelmanta1900 wrote:
had to cull a runt early on... all 5 seeds were female... possibly from a feminized plant...

I didn't know you could tell a seed was female.....I was always told you had to let them grow a bit before you could sex them,and then remove the male plants........or so I heard Whistle
opelmanta1900 Offline
#176 Posted:
Joined: 01-10-2012
Posts: 13,954
Gotta admit, not too worried about keeping them consistent... more just surprised out how different seeds of the same parents could be from one another... one of these plants is literally covered in long white hairs that look like cactus needles... not an orange hair in sight and its like 50 days into what should be a maximum 63 day flowering cycle...

i am considering harvesting one of these and then reverting her back to full time light and making her a mother... I'm told that's something you can do... we'll see...

I've got seeds saved up from over the years... i don't like spending money on them... these came from a batch of alleged gg4... got about 8 seeds from an ounce... they've got gg4 characteristics but i don't think they're authentic...
opelmanta1900 Offline
#177 Posted:
Joined: 01-10-2012
Posts: 13,954
RMAN4443 wrote:
I didn't know you could tell a seed was female.....I was always told you had to let them grow a bit before you could sex them,and then remove the male plants........or so I heard :-"

Your second sentence is correct... your first sentence, you can't tell the gender of a seed by looking at it... but since all 5 of the seeds in question are now full grown females, i think it's safe to say the seeds were female...
danmdevries Offline
#178 Posted:
Joined: 02-11-2014
Posts: 17,451
I wish I lived in a free state.

We did just get Sunday alcohol sales allowed though, so that's progress right?
banderl Offline
#179 Posted:
Joined: 09-09-2008
Posts: 10,153
opelmanta1900 wrote:
Gotta admit, not too worried about keeping them consistent... more just surprised out how different seeds of the same parents could be from one another... one of these plants is literally covered in long white hairs that look like cactus needles... not an orange hair in sight and its like 50 days into what should be a maximum 63 day flowering cycle...

i am considering harvesting one of these and then reverting her back to full time light and making her a mother... I'm told that's something you can do... we'll see...

I've got seeds saved up from over the years... i don't like spending money on them... these came from a batch of alleged gg4... got about 8 seeds from an ounce... they've got gg4 characteristics but i don't think they're authentic...



You can do that, but it's not 100% successful.
danmdevries Offline
#180 Posted:
Joined: 02-11-2014
Posts: 17,451
On the Indiana Sunday alcohol sales note, I have a lot of crazy religious nutbags in my family, and they're on the Facebook.

One post after the announcement was made that the bill passed and was going to governor's desk for signature was going on about how everybody needs to flood the gov's office with phone calls to veto the bill, "It's our only hope at this point". Then went on to add to it once passed that now that Indiana has Sunday booze it's a slippery slope to the gays getting married and the drug addicts buying their marijuanas at the corner store. Then concluded to pray for our children as undoubtedly the number of rapes and murders is going to go up now.
opelmanta1900 Offline
#181 Posted:
Joined: 01-10-2012
Posts: 13,954
banderl wrote:
You can do that, but it's not 100% successful.

Ya, kinda seemed sketchy to me when i was reading about it.... id hate to spend all the time and energy re raising her and then her children only to have it turn out poorly....
danmdevries Offline
#182 Posted:
Joined: 02-11-2014
Posts: 17,451
banderl wrote:
You can do that, but it's not 100% successful.


Just take cuttings from your best plant now. Well, once you have a cuttings propagation setup in place. Clone what you already have.
victor809 Offline
#183 Posted:
Joined: 10-14-2011
Posts: 23,866
danmdevries wrote:
On the Indiana Sunday alcohol sales note, I have a lot of crazy religious nutbags in my family, and they're on the Facebook.

One post after the announcement was made that the bill passed and was going to governor's desk for signature was going on about how everybody needs to flood the gov's office with phone calls to veto the bill, "It's our only hope at this point". Then went on to add to it once passed that now that Indiana has Sunday booze it's a slippery slope to the gays getting married and the drug addicts buying their marijuanas at the corner store. Then concluded to pray for our children as undoubtedly the number of rapes and murders is going to go up now.


don't the religious people drink on sunday? WTF do they think that blood of christ crap is?
banderl Offline
#184 Posted:
Joined: 09-09-2008
Posts: 10,153
opelmanta1900 wrote:
Ya, kinda seemed sketchy to me when i was reading about it.... id hate to spend all the time and energy re raising her and then her children only to have it turn out poorly....



If you let the plant flower too long it probably won't work. The plant just won't have enough energy to come back.

The potency will be the same, however, it might have more of a chance of turning hermi on you
banderl Offline
#185 Posted:
Joined: 09-09-2008
Posts: 10,153
danmdevries wrote:
Just take cuttings from your best plant now. Well, once you have a cuttings propagation setup in place. Clone what you already have.



It's tough to take cuttings when the plant has already flowered. He can try it, but each of those cuttings counts as a plant.
He might as well just try to regenerate his best one
opelmanta1900 Offline
#186 Posted:
Joined: 01-10-2012
Posts: 13,954
Dinnertime... quesadilla burger and watching some YouTube fishermen...
danmdevries Offline
#187 Posted:
Joined: 02-11-2014
Posts: 17,451
banderl wrote:
It's tough to take cuttings when the plant has already flowered. He can try it, but each of those cuttings counts as a plant.
He might as well just try to regenerate his best one


Forgot the plant count thing. Here it don't matter, if caught you get a decade whether it's one or a dozen.

If early in flowering you can get cuttings just fine.
MACS Offline
#188 Posted:
Joined: 02-26-2004
Posts: 79,833
danmdevries wrote:
Transposed your numbers 23 pair 46 total.


Was going to mention that... only reason I knew was from Tool's song 46 and 2. HA!
MACS Offline
#189 Posted:
Joined: 02-26-2004
Posts: 79,833
opelmanta1900 wrote:
Dinnertime... quesadilla burger and watching some YouTube fishermen...


I had pastrami and cheese on dill rye and clam chowdah.
banderl Offline
#190 Posted:
Joined: 09-09-2008
Posts: 10,153
danmdevries wrote:
Forgot the plant count thing. Here it don't matter, if caught you get a decade whether it's one or a dozen.

If early in flowering you can get cuttings just fine.



Early being the important word
danmdevries Offline
#191 Posted:
Joined: 02-11-2014
Posts: 17,451
Applause
MACS wrote:
Was going to mention that... only reason I knew was from Tool's song 46 and 2. HA!

danmdevries Offline
#192 Posted:
Joined: 02-11-2014
Posts: 17,451
Jambalaya and beer.

When I made it, I wasn't thinking about how much it would make.

I probably made 12+ lbs of food.
RMAN4443 Offline
#193 Posted:
Joined: 09-29-2016
Posts: 7,683
MACS wrote:
I had pastrami and cheese on dill rye and clam chowdah.

New England, Manhattan, or Rhode Island? ??
MACS Offline
#194 Posted:
Joined: 02-26-2004
Posts: 79,833
I'll pardon the affront, because you don't really know me... but any chowder with tomatoes in it is not chowder. I like my chowder creamy, with potatoes, lots of clams and NO tomatoes.

fog

(so, yeah... NE)
danmdevries Offline
#195 Posted:
Joined: 02-11-2014
Posts: 17,451
Who TF puts tomatoes in clam chowder?

danmdevries Offline
#196 Posted:
Joined: 02-11-2014
Posts: 17,451
HHD everybody
RMAN4443 Offline
#197 Posted:
Joined: 09-29-2016
Posts: 7,683
MACS wrote:
I'll pardon the affront, because you don't really know me... but any chowder with tomatoes in it is not chowder. I like my chowder creamy, with potatoes, lots of clams and NO tomatoes.

fog

(so, yeah... NE)

Yeah, NE for me too......I knew you were from Rhode Island, so I didn't know if you liked the watery RI style....my dad used to like the Manhattan, but I always hated it.
When we were kids though, we'd go to Rocky Point once or twice a year, and we'd always have lunch in the Food Pavilion, which always included RI style Chowdah and Clam FrittersDrool
I'm assuming you know Rocky Point, what with you being from RIAnxious
8trackdisco Offline
#198 Posted:
Joined: 11-06-2004
Posts: 60,095
19/5.
SmokeMonkey Offline
#199 Posted:
Joined: 04-05-2015
Posts: 5,688
Good morning, y’all
Burner02 Offline
#200 Posted:
Joined: 12-21-2010
Posts: 12,884
Currently 45 and sunny in LA with north wind at 10-15. Increasing morning bike ride from 6.1 to 7.8 miles. Sure the wind will be at a minimum 15 steady when I head out in a few minutes.
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